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At Fluxion, we’re passionate about delivering cell-based and cell-free solutions that facilitate the transformation of research discoveries into new ways to diagnose and treat patients. By characterizing molecular and cellular mechanisms of disease, Fluxion’s platforms help bridge the translational medicine gap, enabling rapid advances in disease research, drug discovery, and the development of diagnostic tests.

1 min read

IonFlux APC Without Compromises

Speaker: Cristian Ionescu-Zanetti PhD, Fluxion Biosciences

Abstract

The IonFlux system is the first automated patch clamp system to achieve high quality giga-seal recordings with continuous perfusion using an affordable and uniquely designed microfluidic substrate.

This webinar will address the following topics:

  • A comparison between single cell, Giga-ohm seal data and ensemble recordings from sets of 20-cells
  • Seal rates and success rates will be discussed for recording a variety of different cell lines
  • Giga-seal data recording (single cell) for a variety of ionic channels will be discussed:
    • Voltage-gated sodium channels (examples using  Nav1.7 and Nav 1.8)
    • Voltage-gated potassium channels (examples using hERG) 
    • Ligand gated channels (examples using ASIC and GABA)

A number of different recording protocols will be presented for Nav 1.8 (a target that traditionally exhibit slow current amplitudes in endogenous systems) and Nav1.7. The protocols will include traditional pharmacology measurements and use dependent block protocols using repeated voltage stimulation.