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At Fluxion, we’re passionate about delivering cell-based and cell-free solutions that facilitate the transformation of research discoveries into new ways to diagnose and treat patients. By characterizing molecular and cellular mechanisms of disease, Fluxion’s platforms help bridge the translational medicine gap, enabling rapid advances in disease research, drug discovery, and the development of diagnostic tests.

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Glycocalyx research utilizing the BioFlux System

The glycocalyx is a covering surrounding the membrane of certain bacteria, epithelia, and other cells. It is composed of glycoproteins and glycolipids, as well as matrix proteins secreted from the cell. In addition to physically protecting the cell, its functions include mediation of cell-cell and cell-ECM interactions. Glycocalyx research has been progressing in recent years, extending from the human cardiovascular system to biofilm research.  The BioFlux system has featured in many of these studies.

Cardiovascular System
The glycocalyx plays a key role in cardiovascular system health, where it serves to protect the vascular wall from direct exposure to blood flow. Disruptions to the glycolalyx can be caused by many factors, including interaction with certain enzymes and proteins, hyperglycemia, and hypoxia. Once degraded, cells at the lumen boundary are subject to shear stress from blood flow, leading to inflammation and atherothrombosis. Research of glycocalyx function is being conducted in several labs using the BioFlux system, which allows researchers to accurately model the physiological environment in the vasculature. Several recent papers include:
Excess sodium is deleterious on endothelial and glycocalyx barrier function: a microfluidic study, Martin, et al 

Acute hyperglycemia exacerbates trauma-induced endothelial and glycocalyx injury: an in vitro model, Diebel, et al 
Glycocalyx serves as a protective layer to surface-attached bacterial colonies, or biofilms, that form on tissue such as the lungs (e.g. Streptococcus pneumoniae) or on inert surfaces such as teeth (e.g. Streptococcus mutans forming dental plaque). The BioFlux system is the leading tool for biofilm research, and has been used extensively in the study of glycocalyx formation, disruption, and methods of prevention. Research papers on this topic include:
Effects of sub-minimum inhibitory concentrations of antimicrobial agents on Streptococcus mutans biofilm formation, Dong et al

Enzymes Catalyzing the TCA- and Urea Cycle Influence the Matrix Composition of Biofilms Formed by Methicillin-Resistant Staphylococcus aureus USA300, De Becker, et al

Contact us to learn more about using BioFlux to accelerate your research.